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<title>Paper Chromatography of Inks</title>
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<p class=3DMsoNormal style=3D'mso-layout-grid-align:none;text-autospace:non=
e'><span
style=3D'font-size:23.0pt;font-family:Arial'>Paper Chromatography of Inks<o=
:p></o:p></span></p>

<p class=3DMsoNormal style=3D'mso-layout-grid-align:none;text-autospace:non=
e'><span
style=3D'font-size:16.0pt;font-family:Arial'>The inks in marking pens are o=
ften
mixtures of dyes of several basic colors. In this <span class=3DSpellE>Mini=
Lab</span>,
you will use the technique of chromatography to analyze the ink from several
pens.<o:p></o:p></span></p>

<p class=3DMsoNormal style=3D'mso-layout-grid-align:none;text-autospace:non=
e'><b
style=3D'mso-bidi-font-weight:normal'><span style=3D'font-size:17.0pt;font-=
family:
Arial'>PURPOSE:</span></b><span style=3D'font-size:17.0pt;font-family:Arial=
'> </span><span
style=3D'font-size:16.0pt;font-family:Arial'>TO USE CHROMATOGRAPHY TO ANALY=
ZE THE
INKS FROM SEVERAL PENS.</span><span style=3D'font-size:17.0pt;font-family:A=
rial'><o:p></o:p></span></p>

<p class=3DMsoNormal style=3D'mso-layout-grid-align:none;text-autospace:non=
e'><b
style=3D'mso-bidi-font-weight:normal'><span style=3D'font-size:17.0pt;font-=
family:
Arial'>HYPOTHESIS: <o:p></o:p></span></b></p>

<p class=3DMsoNormal style=3D'mso-layout-grid-align:none;text-autospace:non=
e'><span
style=3D'font-size:16.0pt;font-family:Arial'><o:p>&nbsp;</o:p></span></p>

<p class=3DMsoNormal style=3D'mso-layout-grid-align:none;text-autospace:non=
e'><b
style=3D'mso-bidi-font-weight:normal'><span style=3D'font-size:17.0pt;font-=
family:
Arial'>MATERIALS: </span></b><span style=3D'font-size:16.0pt;font-family:Ar=
ial'>BEAKER,
WATCH GLASS, COFFEE FILTER, VARIOUS INK PENS, PENCIL, METRIC RULER, H<sub>2=
</sub>O,
PAPER TOWEL<o:p></o:p></span></p>

<p class=3DMsoNormal style=3D'mso-layout-grid-align:none;text-autospace:non=
e'><b
style=3D'mso-bidi-font-weight:normal'><span style=3D'font-size:17.0pt;font-=
family:
Arial'>PROCEDURE</span></b><b style=3D'mso-bidi-font-weight:normal'><span
style=3D'font-size:10.0pt;font-family:Arial'><o:p></o:p></span></b></p>

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<p class=3DMsoNormal style=3D'mso-layout-grid-align:none;text-autospace:non=
e'><b><span
style=3D'font-size:16.0pt;font-family:Arial'>1. </span></b><span
style=3D'font-size:16.0pt;font-family:Arial'>Wear an apron and goggles.<o:p=
></o:p></span></p>

<p class=3DMsoNormal style=3D'mso-layout-grid-align:none;text-autospace:non=
e'><b><span
style=3D'font-size:16.0pt;font-family:Arial'>2. </span></b><span
style=3D'font-size:16.0pt;font-family:Arial'>Obtain a BEAKER that is at lea=
st 6
cm high. From a coffee filter, cut a strip of paper about 2.5 cm wide and a=
bout
2.5 cm longer than the height of the BEAKER.<o:p></o:p></span></p>

<p class=3DMsoNormal style=3D'mso-layout-grid-align:none;text-autospace:non=
e'><b><span
style=3D'font-size:16.0pt;font-family:Arial'>3. </span></b><span
style=3D'font-size:16.0pt;font-family:Arial'>Place the paper strip in the B=
EAKER
so that the bottom of the strip just rests on the bottom of the BEAKER.<o:p=
></o:p></span></p>

<p class=3DMsoNormal style=3D'mso-layout-grid-align:none;text-autospace:non=
e'><b><span
style=3D'font-size:16.0pt;font-family:Arial'>4. </span></b><span
style=3D'font-size:16.0pt;font-family:Arial'>Push a pencil through the top =
of the
paper in such a way that when the pencil rests on the top of the BEAKER, the
paper is suspended with its lower edge just touching the bottom.<o:p></o:p>=
</span></p>

<p class=3DMsoNormal style=3D'mso-layout-grid-align:none;text-autospace:non=
e'><b><span
style=3D'font-size:16.0pt;font-family:Arial'>5. </span></b><span
style=3D'font-size:16.0pt;font-family:Arial'>Prepare several strips in the =
same
way, one strip for each type of marker ink you will analyze.<o:p></o:p></sp=
an></p>

<p class=3DMsoNormal style=3D'mso-layout-grid-align:none;text-autospace:non=
e'><b><span
style=3D'font-size:16.0pt;font-family:Arial'>6. </span></b><span
style=3D'font-size:16.0pt;font-family:Arial'>Using one water-soluble ink ma=
rker
for each strip, draw a narrow, horizontal line across the strip about 2 cm =
up
from the bottom. If possible, include one black or brown marker.<o:p></o:p>=
</span></p>

<p class=3DMsoNormal style=3D'mso-layout-grid-align:none;text-autospace:non=
e'><b><span
style=3D'font-size:16.0pt;font-family:Arial'>7. </span></b><span
style=3D'font-size:16.0pt;font-family:Arial'>Add about 1 cm of water to the=
 BEAKER
and suspend the first strip in the water. The marker line must be above the
water level when the strip is suspended in the BEAKER.<o:p></o:p></span></p>

<p class=3DMsoNormal style=3D'mso-layout-grid-align:none;text-autospace:non=
e'><b><span
style=3D'font-size:16.0pt;font-family:Arial'>8. </span></b><span
style=3D'font-size:16.0pt;font-family:Arial'>Cover the top of the cup loose=
ly
with A WATCH GLASS to reduce evaporation.<o:p></o:p></span></p>

<p class=3DMsoNormal style=3D'mso-layout-grid-align:none;text-autospace:non=
e'><span
style=3D'font-size:16.0pt;font-family:Arial'>Observe and record the effect =
on the
marker line as the water moves up the paper.<o:p></o:p></span></p>

<p class=3DMsoNormal style=3D'mso-layout-grid-align:none;text-autospace:non=
e'><b><span
style=3D'font-size:16.0pt;font-family:Arial'>9. </span></b><span
style=3D'font-size:16.0pt;font-family:Arial'>Remove the strip from the cup =
or
beaker when the water level has risen to just below the pencil. Lay the str=
ip
on a paper towel to dry.<o:p></o:p></span></p>

<p class=3DMsoNormal style=3D'mso-layout-grid-align:none;text-autospace:non=
e'><b><span
style=3D'font-size:16.0pt;font-family:Arial'>10. </span></b><span
style=3D'font-size:16.0pt;font-family:Arial'>Repeat procedures 6 through 9 =
with
each of your marker strips.<o:p></o:p></span></p>

<b style=3D'mso-bidi-font-weight:normal'><span style=3D'font-size:17.0pt;
font-family:Arial;mso-fareast-font-family:"Times New Roman";mso-ansi-langua=
ge:
EN-US;mso-fareast-language:EN-US;mso-bidi-language:AR-SA'><br clear=3Dall
style=3D'page-break-before:always'>
</span></b>

<p class=3DMsoNormal style=3D'mso-layout-grid-align:none;text-autospace:non=
e'><b
style=3D'mso-bidi-font-weight:normal'><span style=3D'font-size:17.0pt;font-=
family:
Arial'>OBSERVATIONS:<o:p></o:p></span></b></p>

<p class=3DMsoNormal style=3D'mso-layout-grid-align:none;text-autospace:non=
e'><span
style=3D'font-size:16.0pt;font-family:Arial'>DRAW AND MEASURE HOW FAR IT PI=
GMENT
MOVED ON EACH CHROMATOGRAPHY STRIP<o:p></o:p></span></p>

<p class=3DMsoNormal style=3D'mso-layout-grid-align:none;text-autospace:non=
e'><span
style=3D'font-size:16.0pt;font-family:Arial'><o:p>&nbsp;</o:p></span></p>

<p class=3DMsoNormal style=3D'mso-layout-grid-align:none;text-autospace:non=
e'><span
style=3D'font-size:16.0pt;font-family:Arial'><o:p>&nbsp;</o:p></span></p>

<p class=3DMsoNormal style=3D'mso-layout-grid-align:none;text-autospace:non=
e'><span
style=3D'font-size:16.0pt;font-family:Arial'><o:p>&nbsp;</o:p></span></p>

<p class=3DMsoNormal style=3D'mso-layout-grid-align:none;text-autospace:non=
e'><span
style=3D'font-size:16.0pt;font-family:Arial'><o:p>&nbsp;</o:p></span></p>

<p class=3DMsoNormal style=3D'mso-layout-grid-align:none;text-autospace:non=
e'><span
style=3D'font-size:16.0pt;font-family:Arial'><o:p>&nbsp;</o:p></span></p>

<p class=3DMsoNormal style=3D'mso-layout-grid-align:none;text-autospace:non=
e'><span
style=3D'font-size:16.0pt;font-family:Arial'><o:p>&nbsp;</o:p></span></p>

<p class=3DMsoNormal style=3D'mso-layout-grid-align:none;text-autospace:non=
e'><span
style=3D'font-size:16.0pt;font-family:Arial'><o:p>&nbsp;</o:p></span></p>

<p class=3DMsoNormal style=3D'mso-layout-grid-align:none;text-autospace:non=
e'><span
style=3D'font-size:16.0pt;font-family:Arial'><o:p>&nbsp;</o:p></span></p>

<p class=3DMsoNormal style=3D'mso-layout-grid-align:none;text-autospace:non=
e'><span
style=3D'font-size:16.0pt;font-family:Arial'><o:p>&nbsp;</o:p></span></p>

<p class=3DMsoNormal style=3D'mso-layout-grid-align:none;text-autospace:non=
e'><span
style=3D'font-size:16.0pt;font-family:Arial'><o:p>&nbsp;</o:p></span></p>

<p class=3DMsoNormal style=3D'mso-layout-grid-align:none;text-autospace:non=
e'><span
style=3D'font-size:16.0pt;font-family:Arial'><o:p>&nbsp;</o:p></span></p>

<p class=3DMsoNormal style=3D'mso-layout-grid-align:none;text-autospace:non=
e'><span
style=3D'font-size:16.0pt;font-family:Arial'><o:p>&nbsp;</o:p></span></p>

<p class=3DMsoNormal style=3D'mso-layout-grid-align:none;text-autospace:non=
e'><span
style=3D'font-size:16.0pt;font-family:Arial'><o:p>&nbsp;</o:p></span></p>

<p class=3DMsoNormal style=3D'mso-layout-grid-align:none;text-autospace:non=
e'><span
style=3D'font-size:16.0pt;font-family:Arial'><o:p>&nbsp;</o:p></span></p>

<p class=3DMsoNormal style=3D'mso-layout-grid-align:none;text-autospace:non=
e'><span
style=3D'font-size:16.0pt;font-family:Arial'><o:p>&nbsp;</o:p></span></p>

<p class=3DMsoNormal style=3D'mso-layout-grid-align:none;text-autospace:non=
e'><span
style=3D'font-size:16.0pt;font-family:Arial'><o:p>&nbsp;</o:p></span></p>

<p class=3DMsoNormal style=3D'mso-layout-grid-align:none;text-autospace:non=
e'><b
style=3D'mso-bidi-font-weight:normal'><span style=3D'font-size:17.0pt;font-=
family:
Arial'>ANALYSIS<o:p></o:p></span></b></p>

<p class=3DMsoNormal style=3D'mso-layout-grid-align:none;text-autospace:non=
e'><b><span
style=3D'font-size:16.0pt;font-family:Arial'>1. </span></b><span
style=3D'font-size:16.0pt;font-family:Arial'>Capillary action is the moveme=
nt of
a liquid upward through small pores that exist in some materials. Did you n=
ote any
evidence of capillary action in this <span class=3DSpellE>MiniLab</span>?<o=
:p></o:p></span></p>

<p class=3DMsoNormal style=3D'mso-layout-grid-align:none;text-autospace:non=
e'><b><span
style=3D'font-size:16.0pt;font-family:Arial'>2. </span></b><span
style=3D'font-size:16.0pt;font-family:Arial'>Does your evidence indicate th=
at any
of the marker inks were composed of more than one pigment?<o:p></o:p></span=
></p>

<p class=3DMsoNormal style=3D'mso-layout-grid-align:none;text-autospace:non=
e'><b><span
style=3D'font-size:16.0pt;font-family:Arial'>3. </span></b><span
style=3D'font-size:16.0pt;font-family:Arial'>Which colors contained the gre=
atest number
of pigments?<o:p></o:p></span></p>

<p class=3DMsoNormal><o:p>&nbsp;</o:p></p>

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